P-125: Assessment The Effect of Equisetum Arvense Alcoholic Extract on IVF Rate, Embryo Quality and Development in STZ-Induced Diabetic Female Mice

Background: Diabetes mellitus is a common metabolic circumstance characterized by high blood glucose level and partial or absolute impairment of insulin secretion. Diabetes in females leads to sexual disorders such as loss of libido, low ovulation rate and disorder in oocyte maturation. Therefore the current study was designed to evaluate the protective effect of Equisetum arvense alcoholic extract (EE) on IVF rate and embryo development in diabetic mice. Materials and Methods: To follow-up the present study, 24 mature female mice were divided into four groups as control-sham (n=6), diabetic (n=6) and diabetic + EE (250 and 500 mg/kg, orally, daily gavage). In order to induce diabetes the STZ (50 mg/kg for 5 days) was administered. Following 30 days of STZ administration the animals were humanity sacrificed and the oocytes were collected by dissecting method following superovulation induction (PMSG, 7.5 IU and hCG, 7.5IU after 48 hours). The oocytes were fertilized by fresh mouse sperm in HTF medium containing 4 mg/ml BSA. The rate of fertilization, two cell embryos, arrested embryos and blastocysts rate was examined in period of 120 hours. Results: The animals in D+EE-administrated groups; especially those in D+EE500, showed remarkably (p<0.05) higher fertilizing success rate in comparison to diabetic group. The D+EE-treated groups had significantly (p<0.05) higher percentage of fertilized oocytes developed until blastocyst stage and remarkably (p<0.05) lower percentage of embryos were blocked in different stages of embryonic development. Conclusion: The animals in D+EE-administrated groups; especially those in D+EE500, showed remarkably (p<0.05) higher fertilizing success rate in comparison to diabetic group. The D+EE-treated groups had significantly (p<0.05) higher percentage of fertilized oocytes developed until blastocyst stage and remarkably (p<0.05) lower percentage of embryos were blocked in different stages of embryonic development.